Sterile, racked pipet tips are available in 10uL, 200uL and 1250uL sizes that fit popular pipettors. Barrier tips, robotic tips, and Ovation pipettor tips are also available.
Plastics and seals
PCR plates (96 or 384 well) are available as well as foil or Microseal B seals for storage. We also have black fluorescence plates for use in a plate reader and Qiagen collection tube racks for sample processing.
PCR 2X mastermix
Several Thermo Scientific PCR mastermixes are provided in 1250uL aliquots. Polymerase options include Maxima Taq, Phire HotStart II and Phusion HotStart II. All are provided with or without green tracking dye included, and all utilize hot start enzymes.
We perform the PCR for you.
Cleanup to eliminate primers and available nucleotide triphosphates in a PCR reaction. Required prior to cycle sequencing, or in between certain steps.
50uL aliquots of BigDye v3.1 are available so you only purchase as much as you need to perform your own cycle sequencing reactions.
PEG-based cleanup solution for your nucleic acid samples.
1mL aliquots of pre-diluted PicoGreen are available for fluorometric quantification of your samples.
Aliquots of solutions required for several DNA extraction methods are available. Methods include sorbitol (good for plants), high molecular weight (tissue-versitile method), and proteinase K (good for tissues). Supplied volumes will enable about 24 samples to be processed.
Cycle sequencing using Applied Biosystems BigDye v3.1 and subsequent cleanup for 3130 or 3730 injection. Requires cleaned PCR product as reaction template. Pricing is available for low- (48 samples, priced per sample) and high-throughput needs (priced per 96 well plate).
Fragment analysis prep
We mix formamide and size standard, add your fluorescently labeled PCR product, and denature your sample for capillary analysis.
Agarose gel analysis
We run a gel of your samples. Required between certain steps.
A single injection on our 4 capillary array 3130 sequencer (sequencing or fragment analysis). Good for working out new protocols or when working with only a few samples.
A single injection on our 96 capillary array 3730xl sequencer (sequencing or fragment analysis). Good for projects where many samples must be assayed.
Nextera XT prep
The gold standard for Illumina sequencing of small genomes, Nextera XT kits take just 1 ng of input DNA and produce randomly fragmented libraries for sequencing on Illumina instruments. You provide the DNA, we do the prep.
Agilent Bioanalyzer chip
Use of a single chip and necessary reagents. Useful for assessing the quality of fragmented DNA for MiSeq sequencing preps and for assessing the quality of RNA extracts prior to cDNA synthesis.
This is used to quantify the clusterable fraction of a prep meant for sequencing on the Illumina MiSeq. Each sample is run in triplicate and must be run simultaneously with a set of standards of known quantity.
PippinPrep size selection service
The PippinPrep can select size ranges with high reproducibility from Illumina library preps. This is especially useful for genomic assemblies or for RADseq libraries.
Illumina MiSeq run
The MiSeq can be run in various configurations. Most of the time it is used for sequencing both ends (paired-end sequencing) of short sequences. A 2x150 run (300 cycle) will read 150 bases from each end of every sequence. Since the 2x150 kit contains "v2" chemistry, you can reasonably expect sequence from 10-15 million clusters. v3 chemistry modes (150 and 600 cycle) will yield 15-25 million sequence clusters.
Prep samples for metagenetic analysis of complex environmental samples. We have primers for 16S (515F/806R or 515Fmod/926R), fungal-specific ITS2 (5.8S_Fun/ITS4_Fun), large subunit rRNA (LR0R/LR3), and AMF-specific small subunit rRNA (WANDA/AML2). Details for these primers can be found here. Custom designs can be requested. Users can prep a small number of samples and wait for a run to fill up or can choose to purchase a full sequencing run in order to receive data more quickly. We currently have 384 separate indexes for amplicon preps. Submitted samples must be PCR quality.
Samples are prepped for RADseq using enzymes EcoRI/MseI. We can use alternative enzymes, though some additional cost may be incurred. Samples submitted for RADseq need to be high quality and purity. We typically require at least 25ng per sample, but have achieved success with as little as 1ng input.
We perform post-laboratory analysis of your data. A consultation is required prior to any data analysis service.